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Forensic Epigenetics, A Novel Method For Body Fluid Identification and Phenotyping

From Feb 28 2018

Diagnostic epigenetic loci are typically located using whole genome array studies which are then followed by quantitative pyrosequencing. Because methylation information is lost following the PCR, extracted samples are subjected to bisulfite modification which converts unmethlated cytosines into thymines following PCR. Pyrosequencing is next used to provide a quantitative estimate of the level or percent methylation at each CpG site.

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